Ideally the examination is conducted in dim ambient light, preferably in a darkened room or stall, to minimize interfering reflections. Basic diagnostic procedure includes Schirmer tear test, Microbiologic sampling, Retroillumination, Pupillary Light Reflexes, Examination of the anterior segment, Ophthalmoscopy, Tonometry, Normal Intraocular Pressure, Gonioscopy, Seidel test, Jones or fluorescein passage test, Tear film break-up time (TFBUT), Rose Bengal test, Swinging flashlight test, Dazzle reflex, Palpebral reflex, Menace response and Behavioral testing of vision.
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