Objectives of staining techniques use in microbiology are to examine the morphology, to classify and identify and to examine the different structure.
- For preparation of bacterial culture smear place a drop of water on a clean glass slide.
- Take loopful culture.
- Make a smear.
- Allow the smear air dry.
- Fix the smear by passing on the flame.
- Types of staining methods are Simple staining methods, Differential staining methods and Special staining method.
- In simple staining method one dye is used and it is used to study the morphology as well as presence or absence of bacteria in smear.
- In differential staining method more than one dye is used to differentiate.
- Special staining method is used to demonstrate the presence of certain bacterial structure like spores, capsules, flagella and metachromatic granules.
For more details please download the attachment- PPT.
